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3. Describe the reaction that incorporates new dNTPs into a growing chain of DNA. 4. Explain kinetic proofreading. 5. Explain how rNTPs are distinguished from dNTPs in DNA polymerase. 6. Distinguish between the two functions of the Mg2+ ions in DNA polymerase. 7. What is the importance of the tyrosine, lysine and arginine amino acids in the finger domain of DNA polymerase? 8. What would be the consequence of loss of function of 3' to 5' exonuclease activity in the palm of DNA polymerase? 9. Describe how the loading of helicase and loading of the sliding clamp are similar and how the processes are different. How do the functions of the two proteins differ? 10. In what way is the loading and function of helicase different between eukaryotes and prokaryotes in replication initiation? 11. Why can we say that helicase does not just have one direction of movement? 12. Describe the steps for movement of DNA through the helicase protein. 13. Describe the cooperative binding of single-stranded binding proteins. 14. Compare DNA pol I and III and primase in prokaryotes with the eukaryotic polymerases described in class. 15. If given an image of activity at the replication fork in prokaryotes, describe the multistep process involving the three polymerases that are active on both strands. 16. Within the replisome, what enzyme activities are stimulated by protein-protein interactions? 17. Describe sequences found at the origin of replication. What proteins bind to these

          3. Describe the reaction that incorporates new dNTPs into a growing chain of DNA.
4. Explain kinetic proofreading.
5. Explain how rNTPs are distinguished from dNTPs in DNA polymerase.
6. Distinguish between the two functions of the Mg2+ ions in DNA polymerase.
7. What is the importance of the tyrosine, lysine and arginine amino acids in the finger domain of DNA polymerase?
8. What would be the consequence of loss of function of 3' to 5' exonuclease activity in the palm of DNA polymerase?
9. Describe how the loading of helicase and loading of the sliding clamp are similar and how the processes are different. How do the functions of the two proteins differ?
10. In what way is the loading and function of helicase different between eukaryotes and prokaryotes in replication initiation?
11. Why can we say that helicase does not just have one direction of movement?
12. Describe the steps for movement of DNA through the helicase protein.
13. Describe the cooperative binding of single-stranded binding proteins.
14. Compare DNA pol I and III and primase in prokaryotes with the eukaryotic polymerases described in class.
15. If given an image of activity at the replication fork in prokaryotes, describe the multistep process involving the three polymerases that are active on both strands.
16. Within the replisome, what enzyme activities are stimulated by protein-protein interactions?
17. Describe sequences found at the origin of replication. What proteins bind to these

3. Describe the reaction that incorporates new dNTPs into a growing chain of DNA.
4. Explain kinetic proofreading.
5. Explain how rNTPs are distinguished from dNTPs in DNA polymerase.
6. Distinguish between the two functions of the Mg2+ ions in DNA polymerase.
7. What is the importance of the tyrosine, lysine and arginine amino acids in the finger domain of DNA polymerase?
8. What would be the consequence of loss of function of 3' to 5' exonuclease activity in the palm of DNA polymerase?
9. Describe how the loading of helicase and loading of the sliding clamp are similar and how the processes are different. How do the functions of the two proteins differ?
10. In what way is the loading and function of helicase different between eukaryotes and prokaryotes in replication initiation?
11. Why can we say that helicase does not just have one direction of movement?
12. Describe the steps for movement of DNA through the helicase protein.
13. Describe the cooperative binding of single-stranded binding proteins.
14. Compare DNA pol I and III and primase in prokaryotes with the eukaryotic polymerases described in class.
15. If given an image of activity at the replication fork in prokaryotes, describe the multistep process involving the three polymerases that are active on both strands.
16. Within the replisome, what enzyme activities are stimulated by protein-protein interactions?
17. Describe sequences found at the origin of replication. What proteins bind to these

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