7. In what order would the following three peptides (described by their amino acid composition) be eluted from a cation-exchange chromatography at a pH of 7? (5pts) Peptide A: Ala 10%, Glu 5%, Ser 5%, Leu 10%, Arg 10%, His 5%, Ile 10%, Phe 5%, Tyr 5%, Lys 10%, Gly 10%, Pro 5%, and Trp 10%. Peptide B: Ala 10%, Glu 10%, Gly 5%, Leu 5%, Asp 10%, Arg 5%, Met 5%, Cys 5%, Tyr 5%, Phe 5%, His 5%, Val 5%, Pro 5%, Thr 5%, Ser 5%, Asn 5%, and Gln 5%. Peptide C: Ala 5%, Val 5%, Gly 10%, Asp 5%, Leu 5%, Arg 5%, Ile 5%, Phe 5%, Tyr 5%, Lys 5%, Trp 5%, Ser 5%, Thr 5%, Glu 5%, Asn 5%, Pro 10%, Met 5%, and Cys 5%.
Added by Patricia S.
Close
Step 1
To do this, we will consider the charged amino acids: Glu, Asp (negatively charged), Arg, Lys, and His (positively charged). Peptide A: - Glu: 5% (negative) - Arg: 10% (positive) - His: 10% (positive) - Lys: 10% (positive) Net charge: 25% positive - 5% negative = Show more…
Show all steps
Your feedback will help us improve your experience
Adi S and 69 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
If a mixture of equal amounts of Peptide A, B, and C in solution at pH=7 was applied to an ion exchange column with positive beads, which peptide or peptides would elute from the column with pH 7.0 buffer? Peptide A: Cys 5%, Val 5%, Gly 10%, Asp 5%, Leu 5%, Arg 5%, Ile 5%, Phe 5%, Tyr 5%, Lys 5%, Trp 5%, Ser 5%, Thr 5%, Glu 5%, Asn 5%, Pro 10%, Met 5%, and 5% Ala. Peptide B: Ser 5%, Trp 10%, Glu 5%, Leu 10%, Arg 10%, His 5%, Ile 10%, Phe 5%, Tyr 5%, Lys 10%, Gly 10%, Pro 5%, and Ala 10%. Peptide C: Gln 5%, Ala 10%, Glu 10%, Gly 5%, Leu 5%, Asp 10%, Arg 5%, Met 5%, Cys 5%, Tyr 5%, Phe 5%, His 5%, Val 5%, Pro 5%, Thr 5%, Ser 5%, and Asn 5% peptide A only, peptide B only, peptide C only, peptides A and B, peptides B and C
Sri K.
1. (a) In what order would the amino acids Lys, His, and Ile be eluted from a carboxymethyl column at pH 6? (b) In what order would Asp, Arg, and Leu be eluted from a diethylaminoethyl column at pH 8? 2. Determine the subunit composition of a protein from the following information: Molecular mass by gel filtration: 180 kD; Molecular mass by SDS-PAGE: 90 kD; Molecular mass by SDS-PAGE with 2-mercaptoethanol: 30 kD and 60 kD 3. You digest a protein with trypsin and CNBr and determine the sequence of the resulting peptides by Edman degradation. What is the sequence of the original protein? Trypsin peptides: Val-Leu-Met-Ala-Gly-Asp-Lys Thr-Ser-Met-Gln-Asn Ser-Glu-His-Arg CNBr peptides: Ala-Gly-Asp-Lys-Thr-Ser-Met Ser-Glu-His-Arg-Val-Leu-Met Gln-Asn
Dominador T.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
Watch the video solution with this free unlock.
EMAIL
PASSWORD