7 suppose you run orange g xylene cyanol methylene blue and...

Name: 7 suppose you run orange g xylene cyanol methylene blue and bromophenol blue in adjacent lanes on a gel when the run is complete based on molecular weight alone what would you expect to be t 92948
Uploaded: 2023-02-12T17:48:29-08:00
Duration: 2 min 10 s
Channel: Sri K
Description: 7 suppose you run orange g xylene cyanol methylene blue and bromophenol blue in adjacent lanes on a gel when the run is complete based on molecular weight alone what would you expect to be t 92948

7. Suppose you run Orange G, Xylene Cyanol, Methylene Blue and Bromophenol blue in adjacent lanes on a gel. When the run is complete, based on molecular weight alone, what would you expect to be the order of the dyes on the gel, starting at the bottom? Dye Molecule Molecular weight Order Orange G 452g mole^ -1 Xylene Cyanol 555g mole^ -1 Methylene Blue 320g mole^ -1 Bromophenol blue 670g mole^ -1 Crystal violet 408gmol - 1

10) Each of the proteins listed below is treated with sodium dodecyl sulfate, boiled in a reducing agent, and separated by electrophoresis on denaturing polyacrylamide gel. Use a low molecular weight tracking dye to determine when the separation is complete. After the separation is complete, stain the proteins with another dye called Coomassie Blue (this stains the proteins a lovely shade of blue). Each protein sample runs in an individual lane on the gel. Draw a picture of the gel after staining and indicate which protein each band on the gel corresponds to. Also, indicate the polarity of the voltage gradient that was applied to the gel (either positive or negative) to achieve the separation of the proteins (15 pts). Myoglobin (16,890 daltons) Hemoglobin (two subunits, each with a molecular weight of 15,500 daltons = subunits, each with a molecular mass of 16,000 daltons). Calculate the mass of myoglobin and hemoglobin. Molecular weight: Denaturation

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