8) In PCR, what happens during the annealing phase?a. Denaturation of DNA template strandsb. base-pairing between primers and template strandsc. Extension of target DNA by Taq polymerased. Exponential accumulation of target DNAe. None of the above
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The main phases are denaturation, annealing, and extension. Show more…
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Which of the following steps are catalysed by Taq polymerase in a PCR reaction? (a) Denaturation of template DNA (b) Annealing of primers to template DNA (c) Extension of primer end on the template DNA (d) All the above
Choose the one answer that fits best. Which statement regarding PCR is NOT correct (videos)? a. Taq polymerase adds nucleotides to the primers and creates a complementary strand of DNA b. Annealing requires increased temperatures c. Repeated cycles of denaturation, annealing and extending DNA strands creates many identical copies of DNA d. PCR is a quick way of using minute quantities of DNA to create millions of copies e. PCR is used to amplify a region of chromosome
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Which of the following is NOT a step in the PCR process? A. DNA Ligase joins the copied fragments together B. Denatured DNA is cooled to allow base-pairing with primers C. Double-stranded DNA is denatured at high temperature D. Taq polymerase synthesizes new DNA strands
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