A boy of 8 months of age who had stopped breastfeeding presents with ongoing repeated infections with encapsulated bacteria (Streptococcus and Haemophilus). The child has virtually no antibodies, and you suspect a B-cell deficiency. Outline how you could use monoclonal antibodies and flow cytometry to confirm your diagnosis. If you are correct, what treatment would you advise? What are three genetic defects that could lead to B-cell deficiency? Why doesn't B-cell deficiency generally show up until a child is a few months old and being weaned?
Added by Debbie J.
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Flow cytometry can identify and quantify different cell types based on specific markers on their surface. In this case, you would look for a decrease in the number of B cells compared to other immune cells. Show more…
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