Question

(a) PCR primers must bind to sequences on either side of the target sequence, on opposite strands. Primer binding site 5' 3' 3' 5' Primer binding site Region of DNA to be amplified by PCR (b) When target DNA is made single stranded, primers bind and allow DNA polymerase to work. 5' 3' 3' 5' Primer Primer 5' 3' 3' 5' Where on the figure would DNA polymerase begin to work on each strand and in which direction would it proceed? It would start at the right end (unbound DNA) on the bottom strand and at the 3' end of the probe on the top; on both strands it would move right to left. It would start at the 3' end of each probe; on the top strand it would move right to left and on the bottom strand left to right. It would start at the free end (left on top strand and right on bottom strand); it would move right to left on the top strand and left to right on the bottom strand. It would start at the left end (unbound DNA) on the top strand and at the 3' end of the probe on the bottom; on both strands it would move left to right.

          (a) PCR primers must bind to sequences on either side of the
target sequence, on opposite strands.
Primer
binding site
5'
3'
3'
5'
Primer
binding site
Region of DNA to
be amplified by PCR
(b) When target DNA is made single stranded, primers bind and
allow DNA polymerase to work.
5'
3'
3'
5'
Primer
Primer
5'
3'
3'
5'
Where on the figure would DNA polymerase begin to work on each strand and in which direction would it proceed?
It would start at the right end (unbound DNA) on the bottom strand and at the 3' end of the probe on the top; on both strands it would move right to left.
It would start at the 3' end of each probe; on the top strand it would move right to left and on the bottom strand left to right.
It would start at the free end (left on top strand and right on bottom strand); it would move right to left on the top strand and left to right on the bottom strand.
It would start at the left end (unbound DNA) on the top strand and at the 3' end of the probe on the bottom; on both strands it would move left to right.

(a) PCR primers must bind to sequences on either side of the
target sequence, on opposite strands.
Primer
binding site
5'
3'
3'
5'
Primer
binding site
Region of DNA to
be amplified by PCR
(b) When target DNA is made single stranded, primers bind and
allow DNA polymerase to work.
5'
3'
3'
5'
Primer
Primer
5'
3'
3'
5'
Where on the figure would DNA polymerase begin to work on each strand and in which direction would it proceed?
It would start at the right end (unbound DNA) on the bottom strand and at the 3' end of the probe on the top; on both strands it would move right to left.
It would start at the 3' end of each probe; on the top strand it would move right to left and on the bottom strand left to right.
It would start at the free end (left on top strand and right on bottom strand); it would move right to left on the top strand and left to right on the bottom strand.
It would start at the left end (unbound DNA) on the top strand and at the 3' end of the probe on the bottom; on both strands it would move left to right.

Added by Dominic H.

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