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1A. Imagine that you had an A260/A280 ratio of 0.8. What would this indicate, relative to a ratio of 1.8? This would indicate that proteins are contaminating the DNA. A 1.8 ratio is what pure DNA ratio. 1B. If you had an A260/A280 reading of 0.8, would the actual DNA concentration likely be higher, or lower, than the value estimated from the A260 reading? 2. Although this spectrophotometer calculates the concentration for you, other spectrophotometers just record the A260. If you diluted a sample of DNA 1/100, and then measured an A260 of 0.2, what was the initial concentration of the DNA stock?

          1A. Imagine that you had an A260/A280 ratio of 0.8. What would this indicate, relative to a ratio of 1.8? This would indicate that proteins are contaminating the DNA. A 1.8 ratio is what pure DNA ratio. 1B. If you had an A260/A280 reading of 0.8, would the actual DNA concentration likely be higher, or lower, than the value estimated from the A260 reading? 2. Although this spectrophotometer calculates the concentration for you, other spectrophotometers just record the A260. If you diluted a sample of DNA 1/100, and then measured an A260 of 0.2, what was the initial concentration of the DNA stock?

1A. Imagine that you had an A260/A280 ratio of 0.8. What would this indicate, relative to a ratio of 1.8? This would indicate that proteins are contaminating the DNA. A 1.8 ratio is what pure DNA ratio. 1B. If you had an A260/A280 reading of 0.8, would the actual DNA concentration likely be higher, or lower, than the value estimated from the A260 reading? 2. Although this spectrophotometer calculates the concentration for you, other spectrophotometers just record the A260. If you diluted a sample of DNA 1/100, and then measured an A260 of 0.2, what was the initial concentration of the DNA stock?

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