Question

A7. Answer BOTH parts (a) Calculate the concentration, purity and yield of DNA from the information below. After purifying DNA, the lab technician prepared a diluted solution of the stock solution (50 ?µl) to analyze on the spectrometer. The technician took 5 µl of the stock and added 45 µl of TE buffer to make DNA solution 1. The technician added 150 µl of TE buffer to DNA solution 1 to make DNA solution 2. DNA solution 2 spectrometric readings are as follows: A260nm = 1.8, A280 = 1.0, and A320 = 1.0. Show all calculations. (6 marks) (b) Briefly explain the relationship between transmittance and absorbance. (4 marks)

          A7. Answer BOTH parts
(a) Calculate the concentration, purity and yield of DNA from the information below. After purifying DNA, the lab technician prepared a diluted solution of the stock solution (50 ?µl) to analyze on the spectrometer. The technician took 5 µl of the stock and added 45 µl of TE buffer to make DNA solution 1. The technician added 150 µl of TE buffer to DNA solution 1 to make DNA solution 2. DNA solution 2 spectrometric readings are as follows: A260nm = 1.8, A280 = 1.0, and A320 = 1.0. Show all calculations.
(6 marks)
(b) Briefly explain the relationship between transmittance and absorbance.
(4 marks)

A7. Answer BOTH parts
(a) Calculate the concentration, purity and yield of DNA from the information below. After purifying DNA, the lab technician prepared a diluted solution of the stock solution (50 ?µl) to analyze on the spectrometer. The technician took 5 µl of the stock and added 45 µl of TE buffer to make DNA solution 1. The technician added 150 µl of TE buffer to DNA solution 1 to make DNA solution 2. DNA solution 2 spectrometric readings are as follows: A260nm = 1.8, A280 = 1.0, and A320 = 1.0. Show all calculations.
(6 marks)
(b) Briefly explain the relationship between transmittance and absorbance.
(4 marks)

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