Acid-Fast Staining: Kinyoun Method A. Results Draw cells from the acid-fast stained slide. Label your drawing to differentiate acid-fast from non-acid-fast cells. M. smegmatis and S. epidermidis (Kinyoun method) B. Short-Answer Questions 1. What makes Mycobacterium resistant to staining? 2. What other bacterial genus is acid-fast? 3. What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary stain in the Ziehl-Neelsen method? 4. What is the secondary stain in both acid-fast stain methods? 5. In the Ziehl-Neelsen acid-fast stain, what is the mordant?
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What makes Mycobacterium resistant to staining? Mycobacterium is resistant to staining due to the presence of a high concentration of mycolic acids in their cell walls, which makes the cell wall impermeable to most stains. Show more…
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