1. [CH-08-01] For acid-fast stain, name the a) Primary dye...

1. [CH-08-01] For acid-fast stain, name the a) Primary dye –; b) Decolorizer –; c) Counterstain –. 2. [CH-08-02] How does the decolorizer in the acid-fast stain differ from the decolorizer in Gram stain? – 3. [CH-08-03] What was the technical mistake made during Acid-fast staining if mixture of acid-fast and non-acid-fast bacteria stained red? – 4. [CH-08-04] Describe morphology, cell grouping, temperature and oxygen requirements for Mycobacterium smegmatis. Use appropriate terminology – 5. [CH-08-05] Name 3 or more pathogenic species of acid-fast bacteria and diseases they cause –

Transcript

00:01 The first question is asking about the name of primary dye in case of acid fast stain.

00:15 And the answer is carbolfucine which is red in color.

00:24 Second is the name of decolorizer in acid fast stain.

00:30 And the answer is acid alcohol which is colorless.

00:39 Third is the counter stain used in acid fast stain and the name of counter stain is methyline blue which is blue in color.

00:59 Now the second part of the question is asking about the difference in decolorizer of gram staining method and the decolorizer of acid fast stain.

01:12 So in case of gram staining method, the decolorizer washes the primary stain, which is the crystal voilator from gram negative bacteria.

01:36 As this bacteria having the thin peptidoglycan layer and after decolorization, the bacteria appears colorless, which after the process of counterstaining with sapphire, appear pink in color.

01:57 On the other hand, gram positive bacteria resist decolorization because they have thick peptidobligin layer and so they retain the blue color even after counterstaining method.

02:20 In case of acid fast staining procedure, the decolorizer washes primary stain which is decarble fucine of non -acid fast cells and so these cells becomes colorless which after counter staining with mithline blue appears blue in color but the acid vast cells again resist decolorization because they have the thick lipid wall and so they appear red in color even after the counter staining procedure.

03:20 The third question is asking about the mistake made in acid fast staining method so that both the acid fast bacteria or the acid fast cells as well as the non acid fast bacteria appears red in color.

03:51 So the step that was skipped is the use of decolorizer.

03:57 As decolorizer retains the red color of acid fast cells and so they will appear red in color but it removes the red color from non -acid acid -past cells and so they appear colorless.

04:23 But since the decolorizer is absent, so both of the cells will now appear red in color in the absence of decolorizer...

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