After cell lysis and centrifugation, which components do you expect to find in the supernatant? Insoluble proteins and cellular components Cellular organelles Solubilized proteins Insoluble proteins, cellular components, and organelles.
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Cell lysis is the process of breaking open cells to release their contents, while centrifugation is a technique used to separate components of a mixture based on their size, shape, density, and viscosity. Show more…
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When purifying proteins from eukaryotic cells, it is a good idea to first separate the organelles. If you are interested in mitochondrial proteins, you don't want to have the proteins from the nucleus or the cytoplasm - so you first separate the mitochondria from the rest of the cell. Same for other cell compartments. Which of these methods you can use for separating organelles early in the purification scheme? -gel filtration -differential centrifugation -ion exchange chromatography -electrophoresis -density-gradient centrifugation
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