All of the following are techniques usually needed in the use of genetic evidence in court cases EXCEPT cloning. careful collection of tissues containing intact DNA sequences. the polymerase chain reaction (PCR). gel electrophoresis of DNA. Question 66 1 pts The technology of genetic engineering has revolutionized the pharmaceutical industry through the ability to make generic drugs the ability to use pig proteins to treat disease the ability to use bacteria cultures to produce mass quantities of human proteins such as human insulin and human growth hormone (HGH) the ability to use cow proteins to treat disease
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7- Name three features that a cloning vector for use in bacteria must contain. 1- 2- 3- 8- Briefly state why heat-stable DNA polymerase from a thermophilic bacterium is used in PCR instead of DNA polymerase from E. coli or humans. 9- How many DNA copies will be produced after 5 cycles of PCR? 10- Genes are evenly distributed throughout the human genome. (true, false) 11- Dark staining chromosome bands are correlated with protein synthesis. (true, false) 12- Stem cells with the maximum flexibility for expression are ____ (embryonic, tissue-specific) in nature. 13- Give two reasons why the drug companies who produce insulin wanted to clone the human insulin gene when they already had a reasonably cheap source of the hormone (pig insulin). 1- 2- 14- Which of the following reactions are known to be carried out by a ribozyme? a) DNA synthesis; b) transcription; c) RNA splicing; d) protein hydrolysis 15- Which of the following pairs of codons might you expect to be read by the same tRNA as a result of wobble? a) CUU & UUU; b) GAU & GAA; c) CAC & CAU; d) AAU & AGU; e) CCU & GCU
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6. What was the primary goal of the Human Genome Project? a. Mapping known disease genes to loci on their chromosomes b. Studying the human proteome c. Sequencing the genomes of humans and common lab organisms d. Mutational analysis of the human genome e. Sequencing the human microbiome 7. A branch of genomics that generates three-dimensional structures from their amino acid sequences is known as a. bioinformatics b. comparative genomics c. structural genomics d. pharmacogenomics e. Pharmacogenetics 8. If two genes are suspected to be linked and a test cross is performed to measure their position on a chromosome, how many cM or Mu are there between the genes based on the data from the following cross? Gg,Rr X gg,rr GR phenotype = 34% gr phenotype = 36% Gr phenotype = 12% gR phenotype = 18% a. 30 cM b. 12 cM c. 70 cM d. 18 cM e. 66 cM 9. Some people are unable to properly metabolize the drug Purinethol, which is given to patients with acute lymphoblastic leukemia; therefore, such patients must be first tested for the associated genetic variant to determine proper dosage of the drug. This scenario best illustrates the use of _____. a. pharmacogenomics b. map-based sequencing c. positional cloning d. annotation of DNA sequences e. comparative genomics 10. After completion of a genome sequencing project, the DNA sequences are analyzed to locate and identify genes in a process called _____. a. automation b. comparative genomics c. physical mapping d. compilation e. annotation
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