Question

1. An experiment was conducted to elucidate the pathway for the biosynthesis of compound X. Four auxotrophs were isolated that could only grow on minimal media if it was supplemented with compound X. These auxotrophs carried mutations that blocked compound X biosynthesis. The genes carrying these mutations were named M1-4. The mutants were then tested for growth on minimal media supplemented with known intermediates in the biochemical pathway and the following data was collected: Supplement added Strain None T W R Compound X Wild-type + + + + + M1 - + - - + M2 - - - - + M3 - + + + + M4 - + - + + a. Based on these results, fill in the blanks in the linear pathway for compound X synthesis from Precursor V. [3 marks] Precursor V -> -> -> -> Compound X b. Indicate the location of M1 – M4 on the pathway. [4 marks]

          1. An experiment was conducted to elucidate the pathway for the biosynthesis of compound X. Four auxotrophs were isolated that could only grow on minimal media if it was supplemented with compound X. These auxotrophs carried mutations that blocked compound X biosynthesis. The genes carrying these mutations were named M1-4. The mutants were then tested for growth on minimal media supplemented with known intermediates in the biochemical pathway and the following data was collected:
Supplement added
Strain None T W R Compound X
Wild-type + + + + +
M1 - + - - +
M2 - - - - +
M3 - + + + +
M4 - + - + +
a. Based on these results, fill in the blanks in the linear pathway for compound X synthesis from Precursor V. [3 marks]
Precursor V -> ________ -> ________ -> ________ -> Compound X
b. Indicate the location of M1 – M4 on the pathway. [4 marks]

1. An experiment was conducted to elucidate the pathway for the biosynthesis of compound X. Four auxotrophs were isolated that could only grow on minimal media if it was supplemented with compound X. These auxotrophs carried mutations that blocked compound X biosynthesis. The genes carrying these mutations were named M1-4. The mutants were then tested for growth on minimal media supplemented with known intermediates in the biochemical pathway and the following data was collected:
Supplement added
Strain None T W R Compound X
Wild-type + + + + +
M1 - + - - +
M2 - - - - +
M3 - + + + +
M4 - + - + +
a. Based on these results, fill in the blanks in the linear pathway for compound X synthesis from Precursor V. [3 marks]
Precursor V -> -> -> -> Compound X
b. Indicate the location of M1 – M4 on the pathway. [4 marks]

Added by Bethany C.

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