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Question: Basis of the Antibiotic Disk Assay In the previous lab, you transferred disks saturated with antibiotics to agar plates that had been spread with bacteria. Exposure to moisture in the agar causes the substances in the disks to diffuse into the agar, resulting in a gradient of antibiotic concentration. Bacteria resistant to the concentration of antibiotic that they encounter will survive, while those susceptible to the concentration of antibiotic they encounter will die. The area in which bacteria are susceptible will appear as a clear area around the disk and is referred to as the zone of inhibition. This assay has been standardized such that, by measuring the diameter of the zone of inhibition, you can determine whether the plated bacteria are resistant or susceptible to a particular antibiotic or something in between. Information for specific antibiotics is provided below. The diagram below shows a plate with four antibiotic disks placed on an E. coli bacterial lawn. The disks are represented by white dots and the zones of inhibition are the dark areas around the disks. Using a ruler, measure the zones of inhibition for each disk in mm. Based on these data, which antibiotic is most effective against E. coli? Explain how you drew your conclusion. (1.5 points) Zone of inhibition measurements: Penicillin Augmentin Gentamicin Bactrim

          Question: Basis of the Antibiotic Disk Assay
In the previous lab, you transferred disks saturated with antibiotics to agar plates that had been spread with bacteria. Exposure to moisture in the agar causes the substances in the disks to diffuse into the agar, resulting in a gradient of antibiotic concentration. Bacteria resistant to the concentration of antibiotic that they encounter will survive, while those susceptible to the concentration of antibiotic they encounter will die. The area in which bacteria are susceptible will appear as a clear area around the disk and is referred to as the zone of inhibition. This assay has been standardized such that, by measuring the diameter of the zone of inhibition, you can determine whether the plated bacteria are resistant or susceptible to a particular antibiotic or something in between. Information for specific antibiotics is provided below. The diagram below shows a plate with four antibiotic disks placed on an E. coli bacterial lawn. The disks are represented by white dots and the zones of inhibition are the dark areas around the disks. Using a ruler, measure the zones of inhibition for each disk in mm. Based on these data, which antibiotic is most effective against E. coli? Explain how you drew your conclusion. (1.5 points) Zone of inhibition measurements:
Penicillin
Augmentin
Gentamicin
Bactrim

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