00:01
Hello students, the given question has in different parts.
00:04
In the first part of the question we have asked what will happen if plates were poured only 2 mm deep instead of standard 4 to 6 mm.
00:13
So the consequences would be that the antibiotics diffusion would be limited.
00:38
This will lead to inaccurate result because the depth of agar affect the diffusion of antibiotics through the agar and the shallow the depth the result will be that the zone of inhibition will be small.
01:04
That's why the result will be inaccurate.
01:08
Next question, in this question we have asked why it is important to dilute solution within 20 minutes.
01:16
So the answer is it is important to dilute within 20 minutes because diluted solutions promptly ensures that the antibiotic retains its full potency and this will provide accurate result because this antibiotics can degrade or become less effective when they are exposed to air, light or any other environmental factors.
01:57
So it is important to dilute them within 20 minutes.
02:01
Next question, in this question we have asked how does this antibiotics get out of disc into the agar.
02:10
So the answer is through diffusion.
02:19
Through diffusion antibiotics come out of disc and creates concentration gradient in agar.
02:26
The antibiotic molecules move from area of high concentration which is around the disc to the area of low concentration that is around the agar...