Briefly describe the basic principle of immunohistochemistry. State four purposes of IHC in diagnostic pathology Briefly explain the effect of the following factors in IHC i. Fixation ii. Antigen retrieval iii. Antibody selection iv. Controls List at least 3 reagents that may be used on formalin fixed, paraffin embedded tissue for enzyme induced epitope retrieval. Differentiate between Direct and indirect methods of IHC reaction. List 2 reagents used to improve intracellular antibody penetration and state their effect. Mention 2 buffer solutions for heat - induced epitope retrieval and 2 enzymes for the enzyme-induced epitope retrieval. List 2 causes each for specific and non-specific background staining. Illustrate how multiple immunofluorescence labelling is performed in IHC technique State the criteria (one each) for the selection of both positive and negative controls in IHC.
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Step 1: Basic principle of immunohistochemistry (IHC): - IHC detects specific antigens in tissue sections using labeled antibodies that bind to the target antigen, visualized by a chromogen, fluorophore, or enzyme reaction, allowing localization and qualitative Show more…
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