Cartilage tissue engineering: (a) Assume your research team has developed a polymer scaffold with similar mechanical properties as cartilage. You are asked to further enhance the biofunctionalization of the polymer scaffold (i.e., allowing chondrocyte attachment and growth on the scaffold). Suggest two methods you would do to achieve this. (0.2pt). (b) After modifying your polymer, you would like to evaluate if the polymer stimulates mesenchymal stem cell (MSC) differentiation into chondrocytes in vitro. Describe two assays you would use and what you would quantify with the assays. (0.2pt) (c) Assume you decided to add allogeneic chondrocytes to your polymer scaffold and test the implant in vivo. Describe how the body would respond to this combinatorial (cells + synthetic biomaterial) implant. Describe the sequence of events in terms of the types of inflammation and types of tissue formation. Specify the types of cells involved in events (0.2pt)
Added by Jesus L.
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Another method would be to incorporate growth factors, such as transforming growth factor-beta (TGF-β) or insulin-like growth factor (IGF), into the scaffold to promote chondrocyte proliferation and differentiation. Show more…
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