cellular respiration can be studied in isolated mitochondria by measuring oxygen
Added by Joshua J.
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This typically involves breaking open the cells (homogenization) and then using differential centrifugation to separate the mitochondria from other cellular components. Show more…
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Oxygen consumption by isolated mitochondria can be measured in a device called an oxygen electrode. Typical data are shown below, where the slope of the trace is a measure of the rate of oxygen consumption. In the experiment shown below, mitochondria were provided with a respiratory substrate (NADH) and inorganic phosphate. Explain the response of the mitochondria to the successive additions of ADP, oligomycin (an inhibitor of ATP synthase) and dinitrophenol.
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Mitochondria isolated from liver cells can be used to study the rate of electron response to a variety of chemicals. The rate of electron transport in transport is measured as the rate of disappearance of O2 from the solution using an oxygen-sensitive electrode: How can using the justify disappearance of oxygen from the solution as measure of electron transport?
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There are several ways to measure cellular respiration experimentally. For example, $\mathrm{CO}_{2}$ and $\mathrm{O}_{2}$ gas sensors measure changes over time in the concentration of carbon dioxide or oxygen, respectively. Design two experiments to test the effects of changing two different variables or conditions (one per experiment) on the respiration of a research organism of your choice.
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