Question

Learning Objective I: Evaluate the importance of biotechnology in modern societies Application question #1 - List four specific applications of biotechnology for the benefit of humans. 1. 2. 3. 4. Learning Objective II: Describe the process of gene extraction. Application Question #2 - Modern biotechnology relies on the study of the properties of DNA and the instructions encoded in the base-pair sequences of chromosomes. In order to study DNA, it must first be extracted from the cells under study. Using numbers 1-5, rank the steps involved in DNA extraction listed below. 1. A. DNA is precipitated using cold ethanol 2. B. Centrifuge cell mix to form a pellet of cellular debris 3. C. Cells are split open (lysed) using detergent solution 4. D. Treat cell contents with protease and RNAse to prevent DNA degradation 5. E. Transfer supernatant solution containing DNA to a clean tube Learning Objective III: Explain how Agarose Gel Electrophoresis and DNA fingerprints are used to compare the differences between DNA samples. Application Question #3 - DNA extracted from cells through the process studied here is sometimes used to decide if two DNA samples are from different people or if they correspond to the same person. DNA fingerprints can be prepared through the process of agarose gel electrophoresis. After reading about this process, write in the missing words or answer the following questions: A. Using an electrical current, DNA fragments move towards the electrode. B. DNA fragments are separated on the basis of size. C. What fragment sizes migrate the longest in the electrophoresis gel? D. Where in the gel do you see uncut or too large DNA molecules? Learning Objective IV: Identify the uses of Polymerase Chain Reaction (PCR) in biology. Application question #4 - Briefly explain, what is the main goal of PCR? Application question #5 - Identify four purposes for the use of PCR in the laboratory: A. B. C. D. Learning Objective V: Compare and contrast the processes of molecular cloning and reproductive cloning. Application Question #6 - Molecular cloning, the production of extra copies of a desired molecule or a particular gene, involves several key procedures and players. Match the components of molecular cloning on the left with their appropriate description on the right. 1. Plasmid A. Unmatched DNA bases at the place where DNA is cut 2. Restriction enzyme B. Molecule used to join DNA fragments 3. Recombinant DNA C. A circular piece of DNA used to introduce genetic material to a bacterial cell. 4. DNA Ligase D. When bacteria take up a plasmid with foreign DNA 5. "Sticky ends" E. Genetic material combining DNA from two parents 6. Bacteria transformation F. Molecule used like "molecular scissors" to cut DNA

Name: chapter 10 review biotechnology learning objectives and application questions learning objectives are identified for you to review your understanding of this topic you are advised to reflect 28426
Uploaded: 2023-09-07T12:29:28-08:00
Duration: 4 min 49 s
Channel: Adi S
Description: chapter 10 review biotechnology learning objectives and application questions learning objectives are identified for you to review your understanding of this topic you are advised to reflect 28426

Learning Objective I: Evaluate the importance of biotechnology in modern societies

Application question #1 - List four specific applications of biotechnology for the benefit of humans.

1.
2.
3.
4.

Learning Objective II: Describe the process of gene extraction.

Application Question #2 - Modern biotechnology relies on the study of the properties of DNA and the instructions encoded in the base-pair sequences of chromosomes. In order to study DNA, it must first be extracted from the cells under study. Using numbers 1-5, rank the steps involved in DNA extraction listed below.

1. A. DNA is precipitated using cold ethanol
2. B. Centrifuge cell mix to form a pellet of cellular debris
3. C. Cells are split open (lysed) using detergent solution
4. D. Treat cell contents with protease and RNAse to prevent DNA degradation
5. E. Transfer supernatant solution containing DNA to a clean tube

Learning Objective III: Explain how Agarose Gel Electrophoresis and DNA fingerprints are used to compare the differences between DNA samples.

Application Question #3 - DNA extracted from cells through the process studied here is sometimes used to decide if two DNA samples are from different people or if they correspond to the same person. DNA fingerprints can be prepared through the process of agarose gel electrophoresis. After reading about this process, write in the missing words or answer the following questions:

A. Using an electrical current, DNA fragments move towards the electrode.
B. DNA fragments are separated on the basis of size.
C. What fragment sizes migrate the longest in the electrophoresis gel?
D. Where in the gel do you see uncut or too large DNA molecules?

Learning Objective IV: Identify the uses of Polymerase Chain Reaction (PCR) in biology.

Application question #4 - Briefly explain, what is the main goal of PCR?

Application question #5 - Identify four purposes for the use of PCR in the laboratory:

A.
B.
C.
D.

Learning Objective V: Compare and contrast the processes of molecular cloning and reproductive cloning.

Application Question #6 - Molecular cloning, the production of extra copies of a desired molecule or a particular gene, involves several key procedures and players. Match the components of molecular cloning on the left with their appropriate description on the right.

1. Plasmid A. Unmatched DNA bases at the place where DNA is cut
2. Restriction enzyme B. Molecule used to join DNA fragments
3. Recombinant DNA C. A circular piece of DNA used to introduce genetic material to a bacterial cell.
4. DNA Ligase D. When bacteria take up a plasmid with foreign DNA
5. "Sticky ends" E. Genetic material combining DNA from two parents
6. Bacteria transformation F. Molecule used like "molecular scissors" to cut DNA

Added by Michele G.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

Instant Answer

Solved by Expert Adi S

09/07/2023

Step 1

Evaluate the importance of biotechnology in modern societies. Application question #1 – List four specific applications of biotechnology for the benefit of humans. Show more…

Show all steps

Thanks for your feedback!

Learning Objective I: Evaluate the importance of biotechnology in modern societies Application question #1 - List four specific applications of biotechnology for the benefit of humans. 1. 2. 3. 4. Learning Objective II: Describe the process of gene extraction. Application Question #2 - Modern biotechnology relies on the study of the properties of DNA and the instructions encoded in the base-pair sequences of chromosomes. In order to study DNA, it must first be extracted from the cells under study. Using numbers 1-5, rank the steps involved in DNA extraction listed below. 1. A. DNA is precipitated using cold ethanol 2. B. Centrifuge cell mix to form a pellet of cellular debris 3. C. Cells are split open (lysed) using detergent solution 4. D. Treat cell contents with protease and RNAse to prevent DNA degradation 5. E. Transfer supernatant solution containing DNA to a clean tube Learning Objective III: Explain how Agarose Gel Electrophoresis and DNA fingerprints are used to compare the differences between DNA samples. Application Question #3 - DNA extracted from cells through the process studied here is sometimes used to decide if two DNA samples are from different people or if they correspond to the same person. DNA fingerprints can be prepared through the process of agarose gel electrophoresis. After reading about this process, write in the missing words or answer the following questions: A. Using an electrical current, DNA fragments move towards the electrode. B. DNA fragments are separated on the basis of size. C. What fragment sizes migrate the longest in the electrophoresis gel? D. Where in the gel do you see uncut or too large DNA molecules? Learning Objective IV: Identify the uses of Polymerase Chain Reaction (PCR) in biology. Application question #4 - Briefly explain, what is the main goal of PCR? Application question #5 - Identify four purposes for the use of PCR in the laboratory: A. B. C. D. Learning Objective V: Compare and contrast the processes of molecular cloning and reproductive cloning. Application Question #6 - Molecular cloning, the production of extra copies of a desired molecule or a particular gene, involves several key procedures and players. Match the components of molecular cloning on the left with their appropriate description on the right. 1. Plasmid A. Unmatched DNA bases at the place where DNA is cut 2. Restriction enzyme B. Molecule used to join DNA fragments 3. Recombinant DNA C. A circular piece of DNA used to introduce genetic material to a bacterial cell. 4. DNA Ligase D. When bacteria take up a plasmid with foreign DNA 5. "Sticky ends" E. Genetic material combining DNA from two parents 6. Bacteria transformation F. Molecule used like "molecular scissors" to cut DNA