Choosing primer locations DNA becomes single stranded during the denaturation phase of PCR. Then the temperature is lowered to allow primers to anneal. The primers will anneal to complementary regions. 5' 3' A C Target sequence B D 3' 5' DNA direction of synthesis Review the "DNA direction of synthesis" diagram and select the primer locations that will amplify the target DNA sequence. 2
Added by Yolanda F.
Close
Step 1
The diagram shows the DNA direction of synthesis. Primers must be positioned so that DNA polymerase can synthesize the target sequence in both directions. Show more…
Show all steps
Your feedback will help us improve your experience
Qbs Educator and 96 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
A. Primers are short pieces of DNA. B. The forward primer sequence is identical to the sense DNA strand (also called top or leading strand), while the reverse primer sequence is complementary and reverse to the sense strand. C. The purpose of the primers is to prime the DNA polymerase. D. Primers bind to the template always at 72ºC. 2. The elongation temperature for the PCR reaction was programmed at 72ºC. Why? A. This is the temperature at which DNA is most stable. B. This temperature is optimal for melting the DNA. C. This temperature is optimal for the polymerase. D. This temperature is optimal for the primers to anneal. 3. To your PCR reaction tube, you added PfuUltra II Fusion HS DNA Polymerase. Why? A. To amplify the DNA. B. To melt the DNA. C. To ensure that the proper gene is amplified. D. To anneal the primers to the DNA template. 4. Jane is setting up a PCR reaction. The genomic DNA she will use has a concentration of 40 ng/µl. She needs about 100 ng of DNA template in the reaction tube. How many µl of the template should she add? A. 4. B. Can't answer the question since the final volume of the PCR reaction is not provided. C. 0.4. D. 2.5. 5. Which one is the correct sequential order of the steps in a PCR reaction? A. Anneal primers, Denature DNA, Elongate DNA. B. Elongate DNA, Anneal primers, Denature DNA. C. Denature DNA, Elongate DNA, Anneal primers. D. Denature DNA, Anneal primers, Elongate DNA.
Qbs E.
Adi S.
(a) PCR primers must bind to sequences on either side of the target sequence, on opposite strands. Primer binding site. Primer binding site. Region of DNA to be amplified by PCR. (b) When target DNA is made single stranded, primers bind and allow DNA polymerase to work. Primer. Primer. Where on the figure would DNA polymerase begin to work on each strand and in which direction would it proceed? It would start at the right end (unbound DNA) on the bottom strand and at the 3' end of the probe on the top; on both strands, it would move right to left. It would start at the 3' end of each probe; on the top strand, it would move right to left and on the bottom strand, left to right. It would start at the free end (left on top strand and right on bottom strand); it would move right to left on the top strand and left to right on the bottom strand. It would start at the left end (unbound DNA) on the top strand and at the 3' end of the probe on the bottom; on both strands, it would move left to right.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD