00:01
Hello students, here we have to find the specific activity yield and purification factor.
00:08
To find specific activity we can use the formula u by mg.
00:12
U is the amount of activity and mg is the amount of specific protein activity.
00:19
So when we calculate these values, first of all at the starting point specific activity yield and purification factor will not be there.
00:29
After that the specific activity will be 0 .3, 0 .5, 2 .4 and 90.
00:45
Yield is calculated by dividing the amount of specific protein activity at each step by the starting amount and then multiplying by 100 and we get the values as 30, 40, 20 and 4.
01:02
Purification factor is calculated by dividing the specific activity at each step by the previous steps specific activity that is 0 .9, 1 .67, 4 .8 and 180.
01:18
To find the cumulative yield which is the cumulative amount of protein recovered at each step.
01:35
So first step that is ammonium sulfate that is 30%.
01:48
In the second step cumulative yield that is ammonium sulfate cut plus gel filtration yield that is 30 plus 40, 70%.
02:06
In third step cumulative yield of iron exchange we will add all those 70 % plus 20 % which is 90%...