00:01
In this question, we have specific activity, specific activity, it is equals to the total enzyme divided by total protein, total enzyme divided by total protein.
00:21
So next we have fold purification, fold purification.
00:29
So this is the specific activity, specific activity divided by the initial specific activity, that is 8 .2 in our case.
00:49
So now next we have percent yield, it is equals to total enzyme activity, total enzyme activity divided by the starting enzyme activity, starting enzyme activity into 100.
01:14
In our case, starting enzyme activity is 4, 2, double, 0.
01:19
So now we have few steps, few steps, first would be the initial starting material, then second we have our purification, purification, then third we have our deat, flow through ph, flow through ph 7 .0, then next we have affinity chromatography, chromatography.
01:59
So then we have protein mg and then next we have enzyme units...