0:00
Hello everyone.
00:01
So, the use of reverse transcriptase to copy rna products into dna.
00:07
Okay.
00:08
So basically we know that we use the reverse, reverse transcriptase, which is an enzyme obviously for the conversion of rna to dna because we know that.
00:33
Dna to rna is known as the transcription.
00:36
Now basically a real time polymerous chain reaction in as a molecular biology laboratory is based on the polymerous chain reaction.
00:48
So basically we find that a real time pcr is done first.
00:57
It analyzes the multiplication of the specific dna molecule.
01:02
So, it analyzes the multiplication of specific of specific dna molecule, molecules even during pcr process rather than the conclusion as in the traditional.
01:34
So, this is even during pcr.
01:42
Okay.
01:44
Then the dna is amplified in three stage.
01:51
Dna is basically amplified in three stages of amplification or as such we can say that this is amplified in three stage of pcr, three stages of p -c -r.
02:13
The first stage is the denaturation.
02:17
There must be a denaturation of the dna.
02:22
Otherwise the strands will not break and will not get the rna...