00:01
You have a circular plasmid pbr322 vector, and it has 4 ,361 base pair long.
00:11
Now the figures above show the cut site for several restriction enzyme along its circular vector molecules.
00:21
So the question says, suppose you cut the vector with the restriction enzyme henry3 and pst1, and then run the digested dna on agarose gel.
00:33
What dna fragment size do you expect to see on gel? so as you can see, i searched all the restriction site along the vector, and i found that pst1 is at nucleotide 3611.
00:51
So this is the number of nucleotide in a vector.
00:54
And you have one pst vector here.
00:57
Now you have a henry3 at 29 of the nucleotide, and they're obviously a arbitrary one here.
01:08
So after you cut with pst1 and henry3, the two enzyme will cut the plasmid twice, once at pst1 site, and then the second during at the henry3 site...