Directions: Use the information in the gel below to determine the sizes of fragments that are present in the gel when the Recombinant Plasmid is digested with the various restriction enzymes. Then, label the plasmid map with the appropriate restriction enzyme cut sites and DNA fragment sizes. Gel: Recombinant Plasmid digested with... Lane 1: Ladder (bp) 6000 3200 2000 1600 1200 800 600 400 300 250 Lane 2: BamH1 Lane 3: HindIII Lane 4: BamH1 & HindIII Determine the sizes of the fragments in the gel when digested with... - BamH1: - HindIII: - BamH1 & HindIII: - Determine the size of the overall plasmid: Complete the drawing of the plasmid map below. ???? ???? b.p. ???? b.p. Recombinant Plasmid ???? b.p. ???? ???? b.p. ????
Added by Lisa J.
Close
Step 1
Step 1: Determine the sizes of the fragments in the gel when digested with BamH1: - Lane 2 (BamH1): 3200 bp - Lane 4 (BamH1 & Hindlll): 1200 bp (fragment from BamH1 digestion) Show more…
Show all steps
Your feedback will help us improve your experience
Shaiju T and 90 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
The diagram below shows the restriction pattern of a plasmid cut with the enzymes BamHI, EcoRI and NdeI. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb BamHI 1.4 10.6 EcoRI 4.5 7.5 NdeI 2.5 9.5 BamHI + EcoRI 1.4 2.7 3.4 4.5 BamHI + NdeI 1.4 2.2 2.5 5.9 EcoRI + NdeI 0.5 2.0 2.5 7.0 (a) Draw an agarose gel with restriction fragments from the data shown above, indicating the size of each fragment. (b) Draw a restriction map showing the fragment sizes on the map.
Madhur L.
Determine the sizes of the uncut plasmid and the restriction fragments produced from the pAdams plasmid using the gel image below. The first lane is the marker which indicates the sizes of pre-known DNA fragments. Use that to indicate where your fragments will be for the 4 samples you prepared (lanes 2-5). Note: bp is number of base pairs Lane 1 Marker Lane 2 Uncut pAdams Lane 3 BamHI only Lane 4 PstI only Lane 5 BamHI + PstI added 4300 bp 4000 bp 3700 bp 3500 bp 3300 bp 3000 bp 2000 bp 1000 bp 500 bp 6. Based on your analysis in Question 5, do you suspect any of the samples of DNA in lanes 3-5 to have the same size of DNA fragment. Describe the evidence that support your conclusion
Farhan A.
Differentiate The plasmid below was cut to produce the five fragments shown in the diagram. The fragments then were separated by gel electrophoresis. Draw a diagram of a gel and the location of each fragment. Label ends as positive or negative. (FIGURE CANT COPY)
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD