CRISPR is: A. Are derived from DNA fragments from viruses B. A family of DNA sequences found within the genomes of prokaryotic organisms C. Shorthand for CRISPR-Cas 9 D. Shorthand for CRISPR-Cas 9, family of DNA sequences found within the genomes of prokaryotic organisms and is derived from DNA fragment from viruses
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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. It is a family of DNA sequences found within the genomes of prokaryotic organisms. Show more…
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2.) What enzyme is used in CRISPR gene editing? Cas3 Cpr9 RNA5 Cas9 3.) What was the original function of the CRISPR system? Defending bacteria from viral infections Defending bacteriophages from bacteria Defending leukocytes from viruses Defending humans from bacteria 4.) CRISPR is A family of DNA sequences derived from viruses in the genome of a bacterial host A danger to the human race An enzyme that can cut at a specific matching target sequence A gene editing tool 5.) Correct order of the life cycle of a bacterial phage: Lysis, Maturation, Penetration, Biosynthesis, Attachment Attachment, Penetration, Biosynthesis, Maturation, Lysis Biosynthesis, Penetration, Attachment, Lysis, Maturation Penetration, Attachment, Lysis, Maturation, Biosynthesis
Marlyn J.
6. (6 points) Match the following components of the CRISPR/Cas9 bacterial immune system with the appropriate description. Each answer is used only once. A. crRNA B. Protospacer C. Spacer D. CRISPR E. Cas9 F. PAM The specific sequence of an invading phage DNA that is cut by the Cas9 endonuclease. Is responsible for the recognition of foreign DNA upon subsequent infection. A sequence in the bacterial genome that is identical to part of the invading phage DNA. The enzyme that destroys phage DNA upon subsequent infection by a foreign DNA. Repeat sequences in the bacterial genome that separate copies of phage DNA sequences. Is the feature on the invading DNA that is required in order for Cas9 to cut.
Supreeta N.
CRISPR (clustered regularly interspaced short palindromic repeats) is a family of DNA sequences found within the genomes of prokaryotic organisms such as bacteria and archaea. These sequences are derived from DNA fragments from viruses that have previously infected the prokaryote and are used to detect and destroy DNA from similar viruses during subsequent infections. Hence, these sequences play a key role in the antiviral defense system of prokaryotes. Cas9 (or "CRISPR-associated protein 9") is an enzyme that uses CRISPR sequences as a guide to recognize and cleave specific strands of DNA that are complementary to the CRISPR sequence. Cas9 enzymes, together with CRISPR sequences, form the basis of a technology known as CRISPR-Cas9 that can be used to edit genes within organisms. (from Wikipedia) 1) How does it work? 2) What is the main advantage of using CRISPR for genome editing compared with the previous best genome-editing tool (called TALENS)? 3) What are its drawbacks?
Sri K.
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