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11. Cyanide ion (CN- ) blocks electron transfer in mitochondria at the level of Complex IV of the ETC. It essentially prevents the formation of peroxide intermediate during H2O formation. 2,4-Dinitrophenol (DNP) is an uncoupler of mitochondrial oxidative phosphorylation - it allows H+ to diffuse across the inner mitochondrial membrane, but without generating ATP. Draw TWO SEPARATE graphs (ATP synthesized vs. time AND O2 consumed vs. time) with labeled curves to show the effects of adding each of these compounds separately to a suspension of mitochondria supplied with O2, succinate, ADP, and Pi. Use an arrow to indicate the time of drug addition. (10 pts)

          11. Cyanide ion (CN- ) blocks electron transfer in mitochondria at the level of Complex IV of the ETC. It essentially prevents the formation of peroxide intermediate during H2O formation. 2,4-Dinitrophenol (DNP) is an uncoupler of mitochondrial oxidative phosphorylation - it allows H+ to diffuse across the inner mitochondrial membrane, but without generating ATP. Draw TWO SEPARATE graphs (ATP synthesized vs. time AND O2 consumed vs. time) with labeled curves to show the effects of adding each of these compounds separately to a suspension of mitochondria supplied with O2, succinate, ADP, and Pi. Use an arrow to indicate the time of drug addition. (10 pts)

11. Cyanide ion (CN- ) blocks electron transfer in mitochondria at the level of Complex IV of the ETC. It essentially prevents the formation of peroxide intermediate during H2O formation. 2,4-Dinitrophenol (DNP) is an uncoupler of mitochondrial oxidative phosphorylation - it allows H+ to diffuse across the inner mitochondrial membrane, but without generating ATP. Draw TWO SEPARATE graphs (ATP synthesized vs. time AND O2 consumed vs. time) with labeled curves to show the effects of adding each of these compounds separately to a suspension of mitochondria supplied with O2, succinate, ADP, and Pi. Use an arrow to indicate the time of drug addition. (10 pts)

Added by Clarence R.

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