Dilution Practice Problem #3 You are given the following bacterial dilution scheme: 1 ml 1 ml 1 ml 1 ml 1 ml A B C D E F Bacterial culture 1 ml 4 ml 9 ml 9 ml 9 ml 5 ml 0.1 ml 0.1 ml 0.1 ml 250 25 5 colonies colonies colonies G H I Individual tube dilutions: A B C D E F Dilution Factor: (=Total tube dilutions) A B C D E F 1. Which plate is the countable plate? (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml. 3. If the original flask had a culture volume of 50ml, how many total cfu's would have been in the original culture?
Added by Jennifer M.
Close
Step 1
First, we need to find the countable plate. The countable range is between 30 and 300 colonies. We are given the following colony counts for each plate: Plate 1: 250 colonies Plate 2: 250 colonies Plate 3: 250 colonies All three plates have countable Show more…
Show all steps
Your feedback will help us improve your experience
Adi S and 70 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: Bacterial culture 4ml 9ml 9ml 9ml 9ml 4ml 0.1 ml 0.1 ml 0.1 ml 750 colonies 75 colonies 15 colonies Individual tube dilutions: Dilution Factor: (=Total tube dilutions) 1. Which plate is the countable plate? (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml. 3. If the original flask had a culture volume of 10.0ml, how many total cfu's would have been in the original culture?
Madhur L.
Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20 mL are added to 80 mL of water. (2) 10 μL from (1) are then added to 9.99 mL of water. (3) A 10^-2 dilution is made from tube # (2). (4) 100 μL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution? b) How many cfu/mL were present in the original culture? Growth Problem. A culture with approximately 2 x 10^3 cells/mL were incubated. After 3 hours, the number of cells had increased to 3 x 10^5. a) How long was the generation time in minutes? b) How many generations have occurred?
Derrick D.
Five milliliters of bacterial culture is added to 45 milliliters of sterile diluent. From this suspension, the following serial dilutions were made: two 1:100 dilutions and one 1:10 dilution. Then, 0.1 milliliters is plated onto Plate Count Agar from the last dilution. After incubation, 186 colonies were counted on the plate. What is the dilution factor, or how much of the original sample is diluted? Calculate the CFU/mL of the original sample.
Sri K.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD