Question

Dilution Practice Problem #5 You are given the following bacterial dilution scheme: 1 ml 1 ml 1 ml 1 ml 1 ml 1 ml Bacterial culture A 4ml B 9ml C 9ml D 9ml E 9ml F 4ml 0.1 ml 0.1 ml 0.1 ml 750 colonies 75 colonies 15 colonies G H I Individual tube dilutions: _ _ _ _ _ _ A B C D E F Dilution Factor: _ _ _ _ _ _ (=Total tube dilutions) A B C D E F 1. Which plate is the countable plate? ________ (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml. 3. If the original flask had a culture volume of 10.0ml, how many total cfu's would have been in the original culture?

          Dilution Practice Problem #5

You are given the following bacterial dilution scheme:
1 ml 1 ml 1 ml 1 ml 1 ml 1 ml
Bacterial culture
A 4ml
B 9ml
C 9ml
D 9ml
E 9ml
F 4ml
0.1 ml 0.1 ml 0.1 ml
750 colonies 75 colonies 15 colonies
G H I

Individual tube dilutions: ___ ___ ___ ___ ___ ___
A B C D E F

Dilution Factor: ___ ___ ___ ___ ___ ___
(=Total tube dilutions) A B C D E F

1. Which plate is the countable plate? ________ (30-300 countable range)

2. Calculate the titer of the original culture in cfu/ml.

3. If the original flask had a culture volume of 10.0ml, how many total cfu's would have been in the original culture?

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Dilution Practice Problem #5

You are given the following bacterial dilution scheme:
1 ml 1 ml 1 ml 1 ml 1 ml 1 ml
Bacterial culture
A 4ml
B 9ml
C 9ml
D 9ml
E 9ml
F 4ml
0.1 ml 0.1 ml 0.1 ml
750 colonies 75 colonies 15 colonies
G H I

Individual tube dilutions:
A B C D E F

Dilution Factor:
(=Total tube dilutions) A B C D E F

1. Which plate is the countable plate? (30-300 countable range)

2. Calculate the titer of the original culture in cfu/ml.

3. If the original flask had a culture volume of 10.0ml, how many total cfu's would have been in the original culture?

Added by Gabriela V.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

Instant Answer

Solved by Expert Madhur L

04/03/2023

Step 1

First, we need to find the countable plate. The countable range is between 30 and 300 colonies. We have the following plates with their respective colony counts: - Plate 1: 750 colonies - Plate 2: 15 colonies - Plate 3: 300 colonies The countable plate is Plate Show more…

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Dilution Practice Problem #5 You are given the following bacterial dilution scheme: Bacterial culture 4ml 9ml 9ml 9ml 9ml 4ml 0.1 ml 0.1 ml 0.1 ml 750 colonies 75 colonies 15 colonies Individual tube dilutions: Dilution Factor: (=Total tube dilutions) 1. Which plate is the countable plate? (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml. 3. If the original flask had a culture volume of 10.0ml, how many total cfu's would have been in the original culture?

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Transcript

00:01 Hello students here we have been given a dilution scheme and we have to tell the dilution in each tube how much dilution is there in each tube so let's work it out tube a we know that in tube a 1 ml is added to 3ml to make it 4m that means the dilution in tube a is equal to 1 force coming to tube b dilution here is we have added 1 ml into 8m.

00:48 Of liquid to make it 9m.

00:54 Total solution so the dilution is 1 upon 9.

01:01 Coming to the tube c dilution here again is c d and e.

01:17 Dilution factor here, not dilution factor but dilution in all these tubes is also 1 upon 9.

01:25 That is 1 9th dilution.

01:27 And coming to the tube f, dilution here is 1 4th because 1ml is added to 3ml to make it 4ml total volume.

01:45 Number second question is here.

01:47 We have to also work out the dilution.

01:52 Factor for each tube.

01:59 So as we know the dilution factor is given by the total volume or final volume after the dilution upon the amount of solution which is added to the diluent.

02:22 That means here in this case 1 .1m.

02:25 Is added which is will be the final volume here.

02:29 Sorry, initial volume.

02:31 Here.

02:36 So dilution factor is given by the final volume which in this case is 4ml and initial volume which in this case is 1m.

02:43 So let's work out dilution factor for each tube...

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