During isoelectric focusing at some point along the pH gradient gel proteins stop moving because the proteins have a net negative charge at that pH. the proteins have a net charge of zero at that pH. the proteins have a net positive charge at that pH. The proteins are not ionized at that pH. their mass is too large at that pH.
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A pH gradient is formed during isoelectric focusing. Which of the following describes how protein molecules distribute themselves during isoelectric focusing? More than one option is correct. Question 5 options: 1. proteins migrate towards the positive electrode at positive pH. 2. proteins migrate in an electric field based on their net charge. 3. proteins cease migration when they have an equal number of positive and negative charges at the ambient pH. 4. proteins migrate in an electric field until pH extremes denature their secondary structure. 5. the isoelectric point of a protein reflects its amino acid composition.
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