00:01
Okay, so this question is asking.
00:03
Basically, we have purified everything necessary for dna replication like we would inside the cell.
00:11
And then when we add the dna and replication was carried out, the nucleotides of the new strands being formed for only a few hundred nucleotides long.
00:22
So which of the following reagents they're asking is not included in the mixture? so what could have been the problem? well, we know that it's not going to be dna polymerase because dna polymerase has worked, right? we get fragments that are a couple, a hundred nucleotides long, but it still works, meaning the dna polymerase is still active.
00:52
Dna ligase still works because the segments are getting added in together.
00:56
So we know dna ligase works.
00:59
Now the next answer is c, nucleotides.
01:04
So it could be that there's not enough nucleotides being added in, but even then typically at that point, maybe, or from personal experience, it seems like if there's not enough nucleotides, you're not going to get any products whatsoever.
01:18
So the answer is going to be the helicase...