4. DILUTION PROBLEMS i. How would you prepare: a. 10 mL of a 1:10 dilution of a 1 M NaCl solution and what would the final concentration of NaCl? b. 80 mL of a 1:20 dilution of a 1 M NaCl solution? c. 50 mL of a 1:25 dilution of a 1 M NaCl solution? ii. How would you prepare exactly 6 mL of a 1/20 dilution (assume the concentration of your starting solution is "1")? iii. You are provided with an antibody solution (Ab) that has a concentration of 600 microgram (?g) / microlitre (?L). For lab, it is necessary to make the following dilutions: a. 10 ?L of 600 ?g/ ? L Ab + 190 ? L of buffer to make a 1:20 dilution at _ ?g/? L b. 20 ? L of 1:20 Ab + 40 ? L of buffer to make a 1:60 dilution at _ ?g/? L c. 5 ? L of 1:60 Ab + 5 ? L of buffer to make a _ dilution at _ ?g/? L d. 10 ? L of 1:60 Ab + 90 ? L of buffer to make a _ dilution at _ ?g/? L e. 10 ? L of 1:60 Ab + 40 ? L of buffer to make a _ dilution at _ ?g/? L f. 10 ? L of 1:60 Ab + 10 ? L of buffer to make a _ dilution at _ ?g/? L iv. How much 2.0 M NaCl solution would you need to make 250 mL of 0.15 M NaCl solution? v. What would be the concentration of a solution made by diluting 45.0 mL of 4.2 M KOH to 250 mL? vi. What would be the concentration of a solution made by adding 250 mL of water to 45.0 mL of 4.2 M KOH? vii. How much 0.20 M glucose solution can be made from 50.0 mL of 0.50 M glucose solution? viii. What is the molarity of a solution that has 4.5 mol of solute dissolved in 300 ml of solution? ix. What is the molarity of a solution of NaOH that has 0.491 g dissolved in 400 ml of solution? x. What is the molarity of a solution prepared by diluting 10.00 ml of a 4.281 M solution to 50.00 ml?
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To prepare 10 mL of a 1:10 dilution of a 1 M NaCl solution, you would need to mix 1 mL of the 1 M NaCl solution with 9 mL of water. The final concentration would be: $$\frac{1\,\text{M}}{10} = \boxed{0.1\,\text{M}}$$ Show more…
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4. DILUTION PROBLEMS i. How would you prepare: a. 10 mL of a 1:10 dilution of a 1 M NaCl solution and what would the final concentration of NaCl? b. 80 mL of a 1:20 dilution of a 1 M NaCl solution? c. 50 mL of a 1:25 dilution of a 1 M NaCl solution? ii. How would you prepare exactly 6 mL of a 1/20 dilution (assume the concentration of your starting solution is "1")? iii. You are provided with an antibody solution (Ab) that has a concentration of 600 microgram (μg) / microlitre (αL). For lab, it is necessary to make the following dilutions: a. 10 μL of 600 μg/ μ L Ab + 190 μ L of buffer to make a 1:20 dilution at _ μg/μ L b. 20 μ L of 1:20 Ab + 40 μ L of buffer to make a 1:60 dilution at _ μg/μ L c. 5 μ L of 1:60 Ab + 5 μ L of buffer to make a _ dilution at _ μg/μ L d. 10 μ L of 1:60 Ab + 90 μ L of buffer to make a _ dilution at _ μg/μ L e. 10 μ L of 1:60 Ab + 40 μ L of buffer to make a _ dilution at _ μg/μ L f. 10 μ L of 1:60 Ab + 10 μ L of buffer to make a _ dilution at _ μg/μ L iv. How much 2.0 M NaCl solution would you need to make 250 mL of 0.15 M NaCl solution? v. What would be the concentration of a solution made by diluting 45.0 mL of 4.2 M KOH to 250 mL? vi. What would be the concentration of a solution made by adding 250 mL of water to 45.0 mL of 4.2 M KOH? vii. How much 0.20 M glucose solution can be made from 50.0 mL of 0.50 M glucose solution? viii. What is the molarity of a solution that has 4.5 mol of solute dissolved in 300 ml of solution? ix. What is the molarity of a solution of NaOH that has 0.491 g dissolved in 400 ml of solution? x. What is the molarity of a solution prepared by diluting 10.00 ml of a 4.281 M solution to 50.00 ml?
Adi S.
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Ronald P.
Calculate the number of moles of solute provided by 50.0 mL of 3.00 M NaOH solution. If 25.0 mL of 0.104 M BaCl2 solution is pipetted into a 100 mL volumetric flask, and water added to the calibration mark, what is the concentration of the resulting solution? If you mix 40.0 mL of a 0.200 M solution of K2CrO4 with an aqueous solution of AgNO3, what mass of solid forms? (Hint: most chromates are insoluble.) Assume you need 50.00 mL of 0.586 M NH4Br (in ethanol). Can you use a 0.837 M solution to make your desired dilute solution? If so, calculate how much of your stock solution you will need, and how much ethanol you will need to add to attain your desired dilution. If not, suggest a method of preparing the desired solution. Calculate the concentration of chloride ions when 100.0 mL of 0.200 M sodium chloride is mixed with 250.0 mL of 0.150 M calcium chloride. In the acid-base neutralization reaction shown below, 1.61 g of the solid acid HC6H5O neutralized 11.61 mL of aqueous NaOH solution. Calculate the molarity of the base solution. NaOH(aq) + HC6H5O(aq) -> H2O(l) + NaC6H5O(aq) Given the reaction below, what amount of Mg(OH)2 (in moles) would be produced by mixing 250.0 mL of 0.240 M NaOH with excess MgCl2? 2NaOH(aq) + MgCl2(aq) -> Mg(OH)2(s) + 2NaCl(aq)
Dj T.
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