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Exercise 6: Theoretical exercise on OD and calibration curve Below you find a table with turbidity measurements (595 nm) of standard dilutions. The protein concentration in the standard stock solution is 100 µg/µl. Blank 1/5 1/10 1/20 1/40 1/80 Curve 1 0.012 0.906 0.440 0.230 0.084 0.072 Curve 2 0.000 0.906 0.440 0.230 0.084 0.072 Curve 3 0.012 0.656 0.334 0.173 0.092 0.052 a. Which of these 3 curves serves as the best calibration curve? Why? b. Calculate the protein concentration for each of the standard dilutions. Concentration (µg/µl) 1/5 1/10 1/20 1/40 1/80 c. Draw the calibration curve. Plot the concentration (µg proteins/µl) on the x-axis and absorbance at 595 nm (A595) on the y-axis. d. Turbidity measurements (at 595 nm) of your sample with unknown concentration are listed below. 1/100 1/200 1/400 Sample 0.571 0.366 0.228 e. Use the calibration curve to determine the concentration of proteins in the undiluted sample. If needed, calculate the mean concentration.

          Exercise 6: Theoretical exercise on OD and calibration curve

Below you find a table with turbidity measurements (595 nm) of standard dilutions. The protein concentration in the standard stock solution is 100 µg/µl.

            Blank    1/5    1/10    1/20    1/40    1/80
Curve 1     0.012   0.906  0.440   0.230   0.084   0.072
Curve 2     0.000   0.906  0.440   0.230   0.084   0.072
Curve 3     0.012   0.656  0.334   0.173   0.092   0.052

a. Which of these 3 curves serves as the best calibration curve? Why?

b. Calculate the protein concentration for each of the standard dilutions.

    Concentration (µg/µl)
    1/5    1/10    1/20    1/40    1/80

c. Draw the calibration curve. Plot the concentration (µg proteins/µl) on the x-axis and absorbance at 595 nm (A595) on the y-axis.

d. Turbidity measurements (at 595 nm) of your sample with unknown concentration are listed below.

          1/100   1/200   1/400
Sample    0.571   0.366   0.228

e. Use the calibration curve to determine the concentration of proteins in the undiluted sample. If needed, calculate the mean concentration.

Exercise 6: Theoretical exercise on OD and calibration curve

Below you find a table with turbidity measurements (595 nm) of standard dilutions. The protein concentration in the standard stock solution is 100 µg/µl.

Blank 1/5 1/10 1/20 1/40 1/80
Curve 1 0.012 0.906 0.440 0.230 0.084 0.072
Curve 2 0.000 0.906 0.440 0.230 0.084 0.072
Curve 3 0.012 0.656 0.334 0.173 0.092 0.052

a. Which of these 3 curves serves as the best calibration curve? Why?

b. Calculate the protein concentration for each of the standard dilutions.

Concentration (µg/µl)
1/5 1/10 1/20 1/40 1/80

c. Draw the calibration curve. Plot the concentration (µg proteins/µl) on the x-axis and absorbance at 595 nm (A595) on the y-axis.

d. Turbidity measurements (at 595 nm) of your sample with unknown concentration are listed below.

1/100 1/200 1/400
Sample 0.571 0.366 0.228

e. Use the calibration curve to determine the concentration of proteins in the undiluted sample. If needed, calculate the mean concentration.

Added by Michael F.

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