the cytoplasm (cyclosis), and the formation of another vacuole begins. Food vacuoles travel in a defined route through the organism (Fig. 16-3). They first pass posteriorly, then anteriorly, and finally posteriorly to the region of the oral groove where the undigestible contents are eliminated through the cytopyge (anal pore). Locate a food vacuole. Observe that when it is first formed, the vacuole is bright red-orange. Closely observe the vacuole as it moves through the protozoan. You will see that during digestion, its contents change from red-orange to blue-green to yellow-green to yellow and finally back to red-orange (Fig. 16-3). The reason for this color change is that Congo red is an indicator dye that changes color with pH: it is blue-green in acidic conditions and red-orange in alkaline conditions. What does this indicate about the pH of the food vacuole as it moves through the organism? The most common type of reproduction in protozoa is binary fission. In this type of asexual reproduction, the cell divides into two genetically identical daughter cells. In flagellates, the plane of division is longitudinal; in ciliates, the parent cell divides transversely. Examine demonstration slides of Paramecium showing the various stages of binary fission (Fig. 16-4). Occasionally, paramecia reproduce sexually by conjugation (Fig. 16-5), in which micronuclei are exchanged. You can observe conjugation in Paramecium by carrying out the following procedure. For this study, you will use mating strains of Paramecium bursaria, which is symbiotic with a green alga. Supplement your observations by examining commercially prepared slides showing various stages of conjugation. Put a small drop of one of the two mating strains into the depression of a deep-well slide. While observing the paramecia with a stereoscopic microscope, add a drop of the second mating strain. You should observe, almost immediately, the agglutination (or clumping) of opposite mating strains, which brings the cells together for the transfer of nuclear material. Place the slide in a covered petri dish containing moist filter paper to prevent dessication of the culture. Examine periodically. Conjugating paramecia can be seen for up to 48 hours, after which few or no conjugants are found.