c) First, test the data against the hypothesis of independent assortment using a chi2 test and expected values based on the assumption of independent assortment in the cross. State your p value, conclusion, and an alternate hypothesis.
Gen. | Obs | Exp. | (O-E)^2/E
--- | --- | --- | ---
B-P- | 103 | 75 | 10.45
B-pp | 49 | 75 | 9.01
bbP- | 44 | 75 | 12.81
bbpp | 104 | 75 | 11.21
3
P VALUE > 0.01
NULL = REJECTED
43.46 = x^2
ALTERNATE HYPOTHESIS IS THAT THE GENES DO NOT SHOW THE GIVEN A.
d) Now test the data against a more reasonable hypothesis, say for instance that the genes are linked and separated by a recombination distance of 25.0 cM; Use the 25.0 cM value to generate expected values for an experimental population of 300 individuals. State your p value and conclusion.
GAMETES = 100%
RECOMB. GAMETES = 25%
PARENTAL GAMETES = 75%
| | bp | 100%
--- | --- | ---
37.5 BP | BPbp | 37.5%
12.5 Bp | Bpbp | 12.5%
12.5 bP | bPbp | 12.5%
37.5 bp | bbpp | 37.5%
e) Now, calculate the linkage of the genes and show a genetic map from the observed data above.