For each of the following stains, list each of the chemicals used (in order), briefly describe what you would observ under the microscope (positive and negative results), and list examples for each. - Gram stain - Acid Fast - Negative Stain - Capsule Stain - Spore stain
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Gram Stain: - Chemicals used (in order): Crystal violet, iodine, alcohol or acetone, and safranin. - Observation under microscope: - Positive result: Gram-positive bacteria will appear purple. - Negative result: Gram-negative bacteria will appear pink or Show more…
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Access the Gram staining procedure simulation with the following link: http://learn.chm.msu.edu/vibl/content/gramstain/gramstain/index.html - After you are finished, list each step for the Gram staining procedure. - Which of the four steps is the most important and why? - What would happen to the cells if you use too much alcohol? - What would happen if you do not use enough alcohol? - What would happen if you left out the application of safranin? For each of the following stains, list each of the chemicals used (in order), briefly describe what you would observe under the microscope (positive and negative results), and list examples for each. - Gram stain - Acid Fast - Negative Stain - Capsule Stain - Spore stain
Dominador T.
2. Complete the table to review what reagent used in each staining: Staining | Primary dye | Mordant if used | decolorizer if used | Secondary dye | Result write the color for | Result Write the color for --- | --- | --- | --- | --- | --- | --- simple | | | | | Direct stain | Negative stain Gram | | | | | Gm Positive | GM Negative Acid fast | | | | | Acid Fast | Non- acid fast (acid fast negative) capsule | | | | | Capsule | No capsule endospore | | | | | Endospore | Vegetative cell
Sri K.
Gram Stain and Acid-Fast Stain. 2. For each of the following steps, indicate what the anticipated color of a Gram-positive or a Gram-negative bacterium would be: Gram stain step Gram-positive bacteria Gram-negative bacteria After crystal violet After Gram's iodine After alcohol After counter-stain (safranin) a. What is the purpose of the iodine? b. Suppose that you are staining an old culture (more than 24 hrs) of Gram-positive organisms. How will the age of the culture affect the staining results? Why?
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