Question

From the human genome sequence, select Blank enzymes that will bracket the region of the huntingtin gene that contains the triplet repeat. Obtain DNADNA from members of families afflicted with Huntington's disease. Carry out Southern blotting with the selected Blank enzymes. Probe each blot with an oligonucleotide ((Blank)n)n or its complement ((Blank)n)n. Or PCR amplify the region of interest and run gel electrophoresis to estimate, from the size of the repeat fragment, the number of repeats; this is what is actually done in HD testing. The age of onset should correlate Blank with the size of the gel band detected by the probe; the larger the band, the Blank the age of onset.

          From the human genome sequence, select Blank enzymes that will bracket the region of the huntingtin gene that contains the triplet repeat. Obtain DNADNA from members of families afflicted with Huntington's disease. Carry out Southern blotting with the selected Blank enzymes. Probe each blot with an oligonucleotide ((Blank)n)n or its complement ((Blank)n)n. Or PCR amplify the region of interest and run gel electrophoresis to estimate, from the size of the repeat fragment, the number of repeats; this is what is actually done in HD testing. The age of onset should correlate Blank with the size of the gel band detected by the probe; the larger the band, the Blank the age of onset.

Added by Kathryn A.

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