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Hello students.
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Today's question is about we want to produce the red floors and protein instead of green floors and protein.
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So we want to produce rfp instead of gfp in this gene, pg -alo gene.
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So we have to use the restriction enzymes to cut the gfp gene prior to ligation.
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So these are the following restriction enzymes.
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They have given us which two combinations we will use.
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So we know that restriction enzymes, restriction enzymes cut at the restriction sites, cut at a specific restriction site.
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Specific restriction site.
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Now we want to remove the gfp.
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Gfp want to remove and we want only rfp, only rfp.
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So we can use those enzymes.
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We can use two enzymes, two enzymes which cut the region flanking the region flanking the gfp protein region.
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Gfp protein region.
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So what does it mean? what does it mean? so suppose gfp, suppose pglo, suppose pglo.
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G -allo gene.
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This is how it look like.
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This is how it look like.
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So suppose there is some area.
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There is some area where we have gfp.
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This is the gfp gene...