How do allolactose and IPTG influence the activity of the repressor in the lac operon
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In the lac operon, the repressor binds allolactose and binds to the promoter, and induces lac gene expression. binds to allolactose and becomes active, blocking lac gene expression. binds to the promoter and blocks lac gene expression. binds to allolactose and becomes inactive, allowing lac gene expression to occur.
Shaiju T.
IPTG can be used in the laboratory as a synthetic inducer of the lac operon, instead of lactose. Using your knowledge of how the lac operon is regulated, predict whether these gene products (̢̢-galactosidase, lactose permease) will be produced (yes (+) or no (-)) in the presence or absence of the inducer IPTG in the following partial diploids:
Suman K.
Suppose you performed a β-galactosidase assay on E. coli with a I+P+OcZ+Y- operon. What results would you expect in the absence and presence of IPTG? In the absence of IPTG, the permease won't be expressed, but β-galactosidase will.
Madhur L.
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