Hydrolysis of p-Nitroacetanilide
Introduction: Give a brief description of the theoretical background for the experiment including the overall reaction, mechanism involved and brief principle behind thin layer chromatography. Remember to state the aim of the experiment.
Results: 1. Calculate the percentage yield of your product using the data below
Mass of empty weighing boat: 1.42 g
Mass of weighing boat + p-nitroacetanilide: 6.44 g
Mass of weighing boat + product: 4.76 g
Questions: Answer the following questions.
1. What is the role of H2SO4 and NaOH in the reaction?
2. What is the stationary phase and mobile phase for the thin layer chromatography.
3. Calculate the Rf values of each spot in the representative TLC plate below and determine the isomer of nitroaniline produced in the experiment.
Note: o = ortho-nitroaniline; m = meta-nitroaniline; p = para-nitroaniline; s = product sample.