00:01
The question that is given over here.
00:01
This is regarding the purity of dna.
00:06
Over here, the purity of dna absorbance extraction is measured by calculating the ratio between the peak of dna and the absorbent peak of common contaminants like protein, which have a peak absorbance at 280 nanometer or chitropic salt which absorbs strongly at 230 nanometer.
00:25
For a dna, a 260 bar a 280 ratio is approximately 1 .8 and 260 by 280 ratio is 1 .8 to 2 .2, which is generally accepted as pure value.
00:38
Over here, we are going to determine a 260 and a 230 od values for dna extraction and based on these value, we are going to identify how pure is our dna extraction.
00:51
They have given that a 230 value is 0 .067, a 260 value is 0 .034, a 280 value is 0 .057.
01:08
So, to determine the purity of dna extraction, let us calculate a 260 by a 280.
01:21
So, this will be 0 .034 divided by 0 .057.
01:29
So, we get 0 .5965 and for a 260 bar a 230...