In a typical PCR reaction, the following stages occur at temperature ranges of 92-95°C. Heating to 92-95°C allows the primers to bind to the denatured DNA. Heating to 92-95°C allows the heat-stable DNA polymerase an opportunity to extend the primers by adding nucleotides to the 3' ends of each growing strand. Heating to 92-95°C denatures the double-stranded DNA so that it dissociates into single strands.
Added by Donald C.
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**Denaturation:** At the temperature range of $92-95^{\circ}C$, the double-stranded DNA denatures, meaning it dissociates into single strands. Show more…
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