In the PCR reaction, you need a three-step reaction cycle, which results in a chain reaction that produces an exponentially growing population of identical DNA molecules. Each step of a reaction cycle is usually performed at a different temperature. a) Name the three steps and state the approximate temperatures at which they take place. (6 marks) b) Using your knowledge of DNA structure, explain the design of these three temperature steps. (6 marks)
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Step 1: Identify the three steps of the PCR reaction cycle and their approximate temperatures. Show more…
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