In last week's experiment, we worked with an enzyme that facilitated a degradation reaction, what is the name of that enzyme, and the substrate that it reacted with? Catalase and Hydrogen Peroxide Catalase and Sucrase Sucrase and Hydrogen Peroxide Sucrase and Sucrose
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In the enzyme lab, we added liver to the hydrogen peroxide. Hydrogen peroxide broke down to produce oxygen gas and water with the use of catalase. Indicate the substrate(s), enzyme(s), and product(s) in this experiment. Substrate(s): Product(s): Enzyme(s): Would catalase enzyme work on lactose? Why or why not?
Keemin L.
During lab experiments with potato catalase (hydrogen peroxidase), you are directed to set up a reaction containing catalase and a solution of sucrose. As you predict, you see no activity in the tube containing these contents. Why is this so?
Sri K.
Now apply this information to preparing for the Catalase Lab. Catalase is an enzyme used by cells to break down hydrogen peroxide a potentially poisonous by-product of many common cellular reactions. Peroxisomes are organelles in the cell that contain catalase, which speeds up the reaction that breaks down hydrogen peroxide (H2O2) into water (H2O) and oxygen (O2). 2 H2O2 ---> 2 H2O + O2 8. The substrate of this reaction = ________________________ WORD AND FORMULA 9. The products of this reaction = ________________ + ________________ WORDS AND FORMULAS By the way, look at the chemical formula above. On the left there are two hydrogen peroxide molecules (2 H2O2). Each one has 2 hydrogens (H2). So there are 2 x 2 = 4 hydrogen atoms in all. On the right side there are 2 molecules of water, each with 2 hydrogens. 2 x 2 = 4 so there are 4 hydrogen atoms. This is called "balancing the equation"; there needs to be the same number of each atom on each side of the equation because you can't destroy or create matter (1st law of thermodynamics) but you can transform it. 10. How many oxygen atoms are on the left side of the equation? ________ Are they the same number as on the right? ________ 11. Draw it out! Draw a cell, with a peroxisome containing catalase. 12. In your drawing, show hydrogen peroxide going in, and the water and oxygen coming out. Label everything.
Josee P.
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