00:01
So in this question we are asked about the factors which affects the pigment chromatography.
00:19
So the chromatography is a separation technique in which there are is one mobile face and other is stationary face.
00:40
And there will be the separation of the solute.
00:51
Now what are the factors? so if we discuss about the factor, the first factor can be the solubility.
01:10
Now see here the experiment.
01:14
So this is the paper.
01:20
This is the paper which is dipped in the mobile phase or we can call it as a solvent and it is tied to this end.
01:28
So when the solute means when the pigment is spotted on this at the particular part, then it will lead to the separation of the pigment.
01:37
So these are the different types of pigment, pigment one, pigment two, pigment three, pigment four.
01:41
Now we can see here the four pigments.
01:44
Example, this is the carotines, xanthofil, chlorophyll, a, chlorophyll, b, and this was the leaf stain...