25. In the Sanger (dideoxy) method for DNA sequencing, a small amount of dideoxynucleotides triphosphate--say, ddCTP--is added to the sequencing reaction along with a larger amount of corresponding dCTP. a. What is the function of the ddCTP and why is it used? b. Why is ddCTP used at much lower concentration compared to the corresponding dCTP? c. What result would be observed if the dCTP were omitted?
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