In the yeast two-hybrid system as illustrated below, which of the following statements accurately completes this sentence: A reporter gene... Gal4-DBD-ProtX Gal4-AD-ProtY X Gal4 Binding Domain DNA-BD Plasmid encodes TRP1 Gal4 BD X -COOH NH2 Y Gal4 Activation Domain AD Plasmid encodes LEU2 Gal4 AD Y -COOH NH2 Gal4 BD X + Gal4 AD Y Y Gal4 AD RNA Pol Gal4 AD Y RNA Pol X Gal4 BD X Gal4 BD Promoter Reporter HIS3 Promoter Reporter HIS3 Schematic of yeast two-hybrid system. BD: Gal4 DNA Binding Domain; AD: Gal4 Activation Domain; RNA Pol: RNA polymerase Select one: a. is fused to the activation domain of a transcription factor. b. is fused to the DNA binding domain of a transcription factor. c. is expressed only if the tested protein interaction between X and Y occurs. d. requires the presence of histidine in the growth medium for its expression.
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Step 1: In the yeast two-hybrid system, the reporter gene Gal4-DBD-Protx Gal4-AD-Proty encodes a fusion protein where Protx is fused to the DNA binding domain (DBD) of the Gal4 transcription factor and Proty is fused to the activation domain (AD) of the Gal4 Show more…
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