Intact mitochondrial were isolated, carefully washed with pH 7.0
buffer, and used to measure the P/O ratio for α-ketoglutarate.
Included in your assay for the measurements were ADP, phosphate
(Pi), NAD+, and malonate in an appropriate buffer. Assume
that all the added compounds can get into the mitochondrial matrix
and that all enzymes of the citric acid cycle are active.
Note that malonate is a competitive inhibitor or succinate
dehydrogenase.
Under these conditions, what would be the P/O ratio for
α-ketoglutarate?
P/O ratio = 0.5
P/O ratio = 1.5
P/O ratio = 2.0
P/O ratio = 3.5
P/O ratio = 5.0