00:01
In this problem, we're asked to identify the properties of an ideal vector.
00:07
And remember, in this chapter, a vector is a plasmid from a bacteria that is used to carry cloned dna into another cell.
00:16
Our plasmid needs to be of the right type to do the genetic engineering that we care about.
00:21
So it could be a t .i.
00:22
Plasmid in plants or plasmid from e.
00:24
Coli.
00:24
If we're doing some bacterial genetic engineering, it needs to have an origin of replication.
00:31
Which is a place where copying or transcription can begin, so this plasmid can copy itself.
00:40
It needs to have a selectable marker region.
00:43
The most common one is ampicillin resistance, but basically some kind of marker that allows us to kill off the bacteria that don't have this plasmid with, for instance, ampicillin.
00:55
It needs a second genetic marker region, which is usually some type of observable phenotype, like a color...