List all the functions of the reagents involved in PCR (Template DNA, dNTPs, Polymerase, Primers, Salt Buffer, Magnesium Ions)
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It provides the necessary genetic information for the synthesis of new DNA strands during the PCR process. Show more…
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Match the components of PCR to their purpose Provides the optimum ionic environment and pH for optimal enzyme activity Pieces of DNA complementary to the template that tell DNA polymerase exactly where to start making copies. Contains the intact sequence of DNA to be amplified. The raw material of newly synthesized DNA. An enzyme that assembles nucleotides into a new DNA chain. Essential cofactor required by DNA polymerase to synthesize the new DNA chain. 1. DNA Template 2. Primers (Oligonucleotides) 3. Magnesium Ions (Mg2+) 4. dNTPs (Deoxyribonucleoside triphosphates) 5. TAQ DNA Polymerase 6. PCR Buffer
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Table 6-2: Functions of PCR Reagents Function Primers What will be copied dNTPs (nucleotides) The machinery that builds the new DNA DNA polymerase The building blocks for the new DNA Template DNA Specifically targets sections of DNA Certain PCR reagents are unique or specific to a PCR. Other reagents are universal, meaning...
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Name four reagents used during a PCR reaction
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